Publications

Current Unpublished Projects

Book

The Cloning, Purification and Derivatization of Recombinant Proteins

Manuscripts

The construction of a Glutamine Sensor: an Advanced Lab Research Project in Biochemistry, in preparation.

Using Knockout Mutants to Investigate the Arginine and Proline Bioynthetic Pathways in E. coli
An Upper Division Laboratory in Microbiology, in preparation.
 

Peer Reviewed Publications


Mechanism of Reverse Transcriptase and DNA Polymerases

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Differential Inhibition of polymerase and Strand-Transfer Activities of HIV-1 Reverse Transcriptase
L.M. Tillekeratne, A. Sherette, J.A. Fulmer, L. Hupe, D. Hupe, S. Gabbara, J.A. Peliska, R.A. Hudson
Bioorg Med Chem Lett., 12(4):525-8 (2002)

Inhibition of HIV-1 Reverse Transcriptase Catalyzed DNA Strand Transfer Reactions by the 4-Chlorophenylhydrazone of Mesoxalic Acid
W. Davis, J. Tomsho, S. Nikam, E.M.Cook, D. Somand and J.A. Peliska
Biochemistry, 46:14279-14291 (2000)

Actinomycin D Inhibition of DNA Strand Transfer Reactions Catalyzed by HIV-1 Reverse Transcriptase and Nucleocapsid Protein
W. Davis, S. Gabbara, D. Hupe and J.A. Peliska
Biochemistry 38: 14213-14221 (1999)

Inhibitors of DNA Strand Transfer Reactions Catalyzed by HIV-1 Reverse Transcriptase
S. Gabbara, W. Davis, L. Hupe, D. Hupe and J.A. Peliska
Biochemistry 38: 13070-13076 (1999)

Use of Fluorescence Resonance Energy Transfer to Investigate the Conformation of DNA Substrates Bound to the Klenow Fragment
S. Furey, C. Joyce, M. Osborn, D. Klenerman, J.A. Peliska, and S. Balasubramanian
Biochemistry, 37: 2979-2990 (1998)

Catalytic Activities Associated With Viral Polymerases
S. Gabbara & J.A. Peliska
Methods in Enzymology 275: 276-310 (1996)

Recombinant HIV-1 Nucleocapsid Protein Accelerates HIV-1 Reverse Transcriptase Catalyzed DNA Strand Transfer Reactions and Modulates RNase H Activity
J.A. Peliska, S. Balasubramanian, D.P. Giedroc, C. Debouck, & S.J. Benkovic
Biochemistry, 33: 13817-13823 (1994)

The Fidelity of In Vitro DNA Strand Transfer Reactions Catalyzed by HIV-1 Reverse Transcriptase
J.A. Peliska & S.J. Benkovic
Biochemistry 33: 3890-3895 (1994)

A Handy Enzyme for Transcription
J.A. Peliska & S.J. Benkovic
Current Biology 2 (10): 521-523 (1992)

HIV-1 Reverse Transcriptase: Spatial and Temporal Relationship Between the Polymerase and RNase H Activities
V. Gopalakrishnan, J.A. Peliska & S.J. Benkovic
Proc. Natl. Acad. Sci. USA 89: 10763-10767 (1992)

Mechanistic Studies of DNA Strand Transfer Reactions Catalyzed By HIV-1 Reverse Transcriptase
J.A. Peliska & S.J. Benkovic
Science 258: 1112-1118 (1992)

Kinetic Characterization of the Polymerase and Exonuclease Activities of the Gene 43 Protein of Bacteriophage T4
T.L. Capson, J.A. Peliska, B.F. Kaboord, M.W. Frey, C. Lively, M. Dahlberg & S.J. Benkovic
Biochemistry 31: 10984-10994 (1992)Mechanism of Reverse Transcriptase and DNA Polymerases

 

Regulation of Nitrogen Assimilation in E. coli

New insights into the mechanism of the kinase and phosphatase activities of Escherichia coli NRII (NtrB) and their regulation by the PII protein.
P. Jiang, A Pioszak, MR Atkinson, JA Peliska and AJ Ninfa.
In Histidine Kinases in Signal Transduction (Inouye, M. and Dutta, R., eds.)
pp. 143–164, Academic Press, New York.(2003)

Asymmetry in the Autophosphorylation of the Two Component Regulatory System Transmitter Protein Nitrogen Regulator II of Escherichia coli.
P. Jiang, J.A. Peliska, & A.J. Ninfa
Biochemistry, 39: 5057-5065 (2000)

Integration of antagonistic signals in the regulation of bacterial nitrogen assimilation
A.J. Ninfa, P. Jiang, M.R. Atkinson & J.A. Peliska
Current Topics Cellular Regulation, Vol 36, 32-75 (2000)

The Regulation of Escherichia coli Glutamine Synthetase Revisited: Role of 2-Ketoglutarate in the Regulation of Glutamine Synthetase Adenylylation State
P. Jiang, J.A. Peliska, & A.J. Ninfa
Biochemistry 37: 12802-12810 (1998)

Reconstitution of the Signal-Transduction Bicyclic Cascade Responsible for the Regulation of Ntr Gene Transcription in Eschericia coli
P Jiang, J.A. Peliska, & A.J. Ninfa
Biochemistry 37: 12795-12801 (1998)

Enzymological characterization of the signal-transducing uridylyltransferase/uridylyl-removing enzyme of Escherichia coli and its interaction with the PII protein
P. Jiang, J.A. Peliska, & A.J. Ninfa
Biochemistry 37: 12782-12794 (1998)


Mechanism of Kinases and Phosphoryl Group Transfers

Preparation and Properties of Enolpyruvate
J.A. Peliska & M.H. O'Leary
J. Am. Chem. Soc. 113:1841-1842 (1991)

Sulfuryl Transfer Catalyzed by Phosphokinases
J.A. Peliska & M.H. O'Leary
Biochemistry 30:1049-1057 (1991)

Synthesis and Study of (Z)-3-Chlorophosphoenolpyruvate
J. Liu, J.A. Peliska & M.H. O'Leary
Arch. Biochem. Biophys. 277:143-148 (1990)

Sulfuryl Transfer Catalyzed by Pyruvate Kinase
J.A. Peliska & M.H. O'Leary
Biochemistry 28:1604-1611 (1989)